Williams’ fine for the code violations, imposed by the United States Tennis Association (USTA), will be deducted from the $1.85 million the American won as the runner-up.
Osaka, 20, won the match 6-2 6-4 to become the first Japanese Grand Slam champion.
Williams, who was aiming to equal Margaret Court’s record of 24 Grand Slam singles titles, refused to shake hands with umpire Ramos after the match.
Williams was given a first code violation after Ramos judged a gesture from coach Patrick Mouratoglou to be coaching.
She said she had not received any tactics from Mouratoglou, telling the umpire she would “never cheat to win and would rather lose”.
The Frenchman later admitted that he had been coaching from the box but that Williams had not seen him doing so.
Williams then received another code violation for a racquet smash at 3-2 in the second set, leading to Ramos docking her a point.