The 23-year-old has a back problem, the Football Association said.
Sterling was the only absentee when the squad reported to St George’s Park on Monday.
No replacement is planned for Sterling, leaving manager Gareth Southgate with a squad of 22 for Saturday’s Uefa Nations League game against Spain and Tuesday’s friendly against Switzerland.
Jamie Vardy and Gary Cahill have effectively retired from international football – although both are available in case of an injury crisis
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